| Repository Information | ||
| Repository Name | ssbd-repos-000159 | |
| Repository URL | http://ssbd.qbic.riken.jp/set/20200716/ | |
| DOI | http://doi.org/10.24631/ssbd.repos.2020.07.002 | |
| Title | Time-lapse images of ERK activity in MDCK cells published in (2020, Boocock et al., Nat Phys) | |
| Description |
Time-lapse images of ERK activity in MDCK cells published in (2020, Boocock et al., Nat Phys). Please see the ""readme.txt"" in each directory for the image conditions. For the experiment conditions, please see the papers (2020, Boocock et al., Nat Phys) and (2020, Hino et al., Dev Cell). "Figure1-ERK_in_confluent" includes the FRET/CFP images for the ERK activity in a confluent condition of MDCK cells. "Figure2-ERK_Response_to_Stretch" includes the FRET/CFP images for the ERK activity in response to the mechanical stretch of MDCK cells. "Figure2-Optogenetic_ERK_Activation" includes a boundary displacement of the cell clusters in response to the optogenetic ERK activation of MDCK cells. "Figure3-ERK_TractionForce" includes the FRET/CFP images for the ERK activity and beads images for the traction force calculation on a polyacrylamide gel. "Figure4-ERK_Wildtype" includes the FRET/CFP images for the ERK activity in the expansion assay of wildtype MDCK cells. "Figure4-ERK_MerlinKO" includes the FRET/CFP images for the ERK activity in the expansion assay of Merlin KO MDCK cells. |
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| License | CC BY license, https://creativecommons.org/license/by/4.0/ | |
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| Contact Information | ||
| Name(s) | Tsuyoshi Hirashima, Edouard Hannezo | |
| E-mail(s) | hirashima.tsuyoshi.2m@kyoto-u.ac.jp, edouard.hannezo@ist.ac.at | |
| Organization | Kyoto University, IST Austria | |
| Department | ||
| Laboratory | ||
| Address | ||
| Imaging Contributors | Tsuyoshi Hirashima, Edouard Hannezo, Naoya Hino, Daniel Boocock, Natalia Ruzickova | |
| Quantitative Data Contributors | ||
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| Method Information | ||
| Method Summary | ||
| Paper DOI | ||
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| Paper information | ||
| Date of publication | None | |
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| Dataset Information | ||
| File Formats | tif | |
| Organism | Canis lupus familiaris | |
| Strain | ||
| Cell Line | Madin-Darby canine kidney cells | |
| Molecular Function (MF) | ||
| Bioligical Process (BP) | ||
| Cellular Component (CC) | ||
| Study Type | ||
| Imaging Methods | Widefield fluorescence microscope | |