| Project Information |
| Project Name |
ssbd-repos-000157 |
| Project URL |
http://ssbd.qbic.riken.jp/set/20200603/ |
| DOI |
http://doi.org/10.24631/ssbd.repos.2020.06.003
|
| Title |
Raw data of an article “Multiple types of navigational information are diffusely and independently encoded
in the population activities of the dentate gyrus neurons” (Murano et al., 2020) |
| Description |
The dentate gyrus (DG) plays critical roles in cognitive functions such as learning, memory, and spatial
coding, and its dysfunction is implicated in various neuropsychiatric disorders. However, it remains largely
unknown how information is represented in this region. Here, we recorded neuronal activity in the DG using
Ca2+ imaging in freely moving mice and analysed this activity using machine learning. Although each individual
neuron was weakly and diversely tuned to multiple information types, the activity patterns of populations of
DG neurons enabled us to successfully decode position, speed, and motion direction in an open field as well as
current and future location in a T-maze. In αCaMKII heterozygous knockout mice, an animal model of
neuropsychiatric disorders, including intellectual disability and bipolar disorder, the decoding accuracy of
position in the open field and future location in the T-maze were selectively reduced. These results suggest
that multiple types of information are diffusely and independently distributed in DG neurons. |
| License |
CC BY license https://creativecommons.org/licenses/by/4.0/ |
|
| Contact Information |
| Name |
Tomoyuki Murao, Ryuichi Nakajima, Tsuyoshi Miyakawa |
| E-mail |
miyakawa@fujita-hu.ac.jp |
| Organization |
Fujita Health University |
| Department |
Institute for Comprehensive Medical Science |
| Laboratory |
Division of Systems Medical Science, Laboratory for Developmental Dynamics |
| Address |
1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi 470-1192, Japan |
| Imaging Contributors |
Ryuichi Nakajima |
| Quantitative Data Contributors |
Tomoyuki Murano, Ryuichi Nakajima |
|
| Method Information |
| Method Summary |
See details in Murano T, et. al. (2020) BioRxiv. |
| Paper DOI |
https://doi.org/10.1101/2020.06.09.141572 |
| Paper URL |
https://www.biorxiv.org/content/10.1101/2020.06.09.141572v2 |
| Paper infomation |
Multiple types of navigational information are diffusely and independently encoded in the population
activities of the dentate gyrus neurons,
Tomoyuki Murano, Ryuichi Nakajima, Akito Nakao, Nao Hirata, Satoko Amemori, Akira Murakami, Yukiyasu Kamitani,
Jun Yamamoto, Tsuyoshi Miyakawa,
bioRxiv 2020.06.09.141572; doi: https://doi.org/10.1101/2020.06.09.141572
|
| Date of Publication |
2020/6/9 |
|
| Dataset Information |
| File Formats |
Raw and processed calcium fluorescence data in the original format of nVista system (inscopix; *.isxd files, paired with *.isxp metafile), count of active cell population (*.xlsx), extracted calcium signal traces of each neuron (*.csv), and cell countour images (*.tiff) are registered. The [*.isxd] data stored in a folder (*_data) are linked to a metafile (*.isxp) located in their parent folder, and these files can be opened with “Inscopix Data Processing Software‘’ available from inscopix (https://www.inscopix.com/). Processed datasets and codes used for the decoding analyses are available at GitHub (https://github.com/tmurano). |
| Organism |
Mus musculus |
| Strain |
C57BL/6J |
| Cell Line |
NA |
| Molecular Function(MF) |
The mutant grpoup of the mice which we studied have heterozygous knockout of a gene coding calcium/calmodulin dependent protein kinase II alpha (CaMKIIa) protein, whose function is involved in synaptic plasticity, neurotransmitter release and long-term potentiation. These mutant mice is an animal model of neuropsychiatric disorders, including intellectual disability and bipolar disorder. |
| Biological Process(BP) |
In the heterozygous CaMKIIa knockout mice, Calbindin, a marker of mature neurons in the dentate gyrus, is largely reduced. The neurons of these mutants had multiple features of normal immature neurons, at molecular, morphological and electrophysiological levels. |
| Cellular Component(CC) |
NA |
| Study Type |
in vivo, calcium imaging, navigational information, spatial coding, dematuration |
| Imaging Methods |
in-vivo, freely behaving, epi-fluorescent calcium imaging, miniature microscope |