Summary of datasets

repository Information
repository Name ssbd-repos-000149
repository URL
Title Live imaging data of developing hair follicles in an article “Origin and induction process of hair follicle stem cells” (Morita et al., 2020).

Tissue stem cells (SCs) are generated from an embryonic progenitor population through organ-specific morphogenetic events. Although tissue SCs are central to organ homeostasis and regeneration, it remains unclear how they are induced during development, mainly owing to the lack of specific markers that exclusively label prospective SCs. Here, by combining marker-independent long-term three-dimensional (3D) live imaging and single-cell transcriptomics, we captured cellular dynamics, dynamic cell lineages, and transcriptome changes in the entire epidermis of developing mouse hair follicles (HFs). We found that different epidermal lineage precursors were aligned in a two-dimensional (2D) concentric manner in the basal layer of the hair placode. Each concentric ring zone acquired unique transcriptomes and telescoped out to form longitudinally aligned 3D cylindrical compartments. Prospective bulge SCs were derived from the peripheral ring zone of the placode, irrespective of cell division orientation, but not from previously suggested suprabasal cells. We also identified 13 gene clusters in which their ensemble expression dynamics drew the entire transcriptional landscape of epidermal lineage diversification, coinciding with cell lineage data. Combining these findings with insect appendage development, we provide a generalized model termed the “telescope model” wherein 2D concentric zones in the placode telescope out to form 3D longitudinally aligned cylindrical compartments.

License CC BY license,

Contact Information
Name(s) Ritsuko Morita, Hironobu Fujiwara
Organization RIKEN
Department Center for Biosystems Dynamics Research
Laboratory Laboratory for Tissue Microenvironment
Address 2-2-3 Minatojima-minamimachi, Chuo-ku, Kobe 650-0047 Japan
Imaging Contributors Ritsuko Morita, Hironobu Fujiwara
Quantitative Data Contributors

Method Information
Method Summary

Paper DOI
Paper URL
Paper information
Date of publication None

Dataset Information
File Formats Raw data (*.oif, *.oir), Processed data (*.ims). Processed data were generated by concatenating and aligning the divided raw data as needed.
Organism Mus musculus
Cell Line
Molecular Function (MF)
Bioligical Process (BP)
Cellular Component (CC)
Study Type
Imaging Methods