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Summary of BDML data

BDML ID194e8520-0042-4923-aa6a-29416053ce5e (schema: 3.000, status: inactive)
TitleBDML file of the PAR-2 trajectories obtained from time-lapse semi-TIRF microscopy images of fluorescent particles of GFP::PAR-2 in a C. elegans pkc-3(RNAi)embryo
LicenseCC BY license
DescriptionThe PAR-2 trajectories obtained from time-lapse semi-TIRF microscopy images of fluorescent particles of GFP::PAR-2 in a C. elegans pkc-3(RNAi)embryo
OrganismC. elegans
Datatypesingle-molecule dynamics
Local IDwtPAR-2pkc-3
Basedon Experiment
Contributors Yukinobu Arata, Michio Hiroshima, Chan-Gi Pack, Ravikrishna Ramanujam, Fumio Motegi, Kenichi Nakazato, Yuki Shindo, Paul Wiseman, Hitoshi Sawa, Tetsuya Kobayashi, Hugo Brandao, Tatsuo Shibata, Yasushi Sako
PubMed ID27524610
Contact InformationYukinobu Arata, RIKEN, Wako, Cellular Informatics Laboratory: Yasushi Sako, RIKEN, Wako, Cellular Informatics Laboratory
Summary of methodsSee details in Arata et al. (2016) Cell Rep. 16, 1-13. The original move of wtPAR-2/pkc-3 (tiff format) is rotated at 135 degree and processed through Rolling Ball and Kalman filters to obtain wtPAR-2pkc-3_RBKL.avi. The PAR-2 trajectories were obtained from the avi file. Kalman filter slightly reduced the diffusion coefficient.

Summary of Images

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