0eb03d7c-cd2a-4198-bb0f-49fa43f629bd
File of the procedures for data processing to obtain cell division dynamics
1
2013-06-11
Method for obtaining cell division dynamics
Koji Kyoda, Shugo Hamahashi, Mitsuru Urai, and Shuichi Onami
Shuichi Onami
sonami@riken.jp
http://so.qbic.riken.jp
RIKEN
Quantitative Biology Center
Laboratory for Developmental Dynamics
Kobe 650-0047, Japan
We first automatically detected nuclear regions (step order=1).
We next manually corrected some errors (step order=2).
automatic detection of nuclear regions
nuclear_detection
http://so.qbic.riken.jp/nuclear_detection/
Use of nuclear_detection.tar.bz2 with a little modification. list of parameters, window_size: 13x13; binary_threhold: 145; snakes_parameters: 0.9, 0.9, 0.9
manual correction of some errors
remove false-positives editor
1.6
Removed false-positive information was saved as .fp file.
Lastly, we identified the trajectory of nuclear division dynamics.
identification of the trajectory
CDP Browser
Log information was saved as .mop file.