f1692365-42e7-4236-986a-5a1bae97420f 2017-10-03

Method for obtaining cell division dynamics Koji Kyoda Shugo Hamahashi Mitsuru Urai Shuichi Onami

Shuichi Onami sonami@riken.jp http://so.qbic.riken.jp RIKEN Quantitative Biology Center Laboratory for Developmental Dynamics

Kobe 650-0047, Japan

We first automatically detected nuclear regions (step order=1). We next manually corrected some errors (step order=2). Lastly, we identified the trajectory of nuclear division dynamics. automatic detection of nuclear regions nuclear_detection http://so.qbic.riken.jp/nuclear_detection/ Use of nuclear_detection.tar.bz2 with a little modification. list of parameters, window_size: 13x13; binary_threhold: 145; snakes_parameters: 0.9, 0.9, 0.9 manual correction of some errors remove false-positives editor Removed false-positive information was saved as .fp file. identification of the trajectory CDP Browser Log information was saved as .mop file.