8f94272d-6ede-4a57-b7ca-02e4604fbcab 2017-10-03

Method for obtaining single molecule division dynamics Takeshi Jin

Takashi Jin tjin@riken.jp RIKEN Quantitative Biology Center Laboratory for Nano-Bio Probes

6-2-3 Furuedai, Suita, Osaka 565-0874, Japan

We first automtically detected and tracked fluorescent spots (step order=1). We manually corrected some errors (step order=2). extraction and tracking of fluorescent spot G-Track ver 1.2 http://www.g-angstrom.com/products/gtrack.php Automatically recognizes all the fluorescent spots in a movie. All position and tracking information of the fluorescent spots were obtained by by Gaussian fitting mode. List of parameters: iteration number for iteration fitting algorithm, ROI size, minimum number of frames for tracing, fluorescent intensity threshold, frame number, and fluorescent intensity (absolute values and background subtraction)) manual correction of some errors remove false-positives editor Removed false-positive information was saved.